In this lab students will understand the action and specificity of restriction enzymes, while learning the components involved in the process of DNA electrophoresis and the role of each component as it applies to the entire process of electrophoresis. Students will perform the electrophoresis process on DNA samples treated with different restriction enzymes and construct a standard curve using a known DNA sample while determining the approximate size of the DNA fragments in unknown samples. Materials provided for eight lab groups.
Kit Includes:
200mL Prepared Agarose, 0.8%
500mL TBE buffer, 5X
60mL DNA Stain, 20X DNA Samples, 100ul each:
![Biotechnology: Restriction Enzyme analysis of DNA, 1022407 [W55007], Electrophoresis Experiments](/imagelibrary/W55007/W55007_01_Biotechnology-Restriction-Enzyme-analysis-of-DNA.jpg)
![Biotechnology: Restriction Enzyme analysis of DNA, 1022407 [W55007], Electrophoresis Experiments](/thumblibrary/W55007/W55007_01_1200_1200_Biotechnology-Restriction-Enzyme-analysis-of-DNA.jpg)
![DC Power Supply 0 – 300 V, 0 – 400 mA, 1010263 [W19926], Electrophoresis Experiments](/thumblibrary/W19926/W19926_01_140_140_DC-Power-Supply-0-300-V-0-400-mA.jpg)
![Electrophoresis Chamber S, 1012852 [W19925], Electrophoresis Experiments](/thumblibrary/W19925/W19925_01_140_140_Electrophoresis-Chamber-S.jpg)
![Microlitre Pipette, 0.5 – 10 µl, fully autoclavable, 1022707 [W161891], Pipets and Micropipets](/thumblibrary/W161891/W161891_01_140_140_Microlitre-Pipette-05-10-µl-fully-autoclavable.jpg)
![Pipette Tips, Crystal, up to 10 µl, 1013424 [W16193], Pipets and Micropipets](/thumblibrary/W16193/W16193_01_140_140_Pipette-Tips-Crystal-up-to-10-µl.jpg)
![DNA Paternity Testing Electrophoresis Lab Activity, 1022426 [W55026], Electrophoresis Experiments](/thumblibrary/W55026/W55026_01_140_140_DNA-Paternity-Testing-Electrophoresis-Lab-Activity.jpg)
![DNA fingerprinting Electrophoresis Lab Activity, 1022427 [W55027], Electrophoresis Experiments](/thumblibrary/W55027/W55027_01_140_140_DNA-fingerprinting-Electrophoresis-Lab-Activity.jpg)
![Microlitre Pipette, 100 – 1000 µl, fully autoclavable, 1022710 [W161921], Pipets and Micropipets](/thumblibrary/W161921/W161921_01_140_140_Microlitre-Pipette-100-1000-µl-fully-autoclavable.jpg)
![Electrophoresis and DNA Fragment Length Determination Lab Activity, 1022425 [W55025], Electrophoresis Experiments](/thumblibrary/W55025/W55025_01_140_140_Electrophoresis-and-DNA-Fragment-Length-Determination-Lab-Activity.jpg)
![AB0/Rh Blood Typing, 1022412 [W56621], Anatomy and Physiology Experiments](/thumblibrary/W56621/W56621_01_140_140_AB0Rh-Blood-Typing.jpg)
![Microlitre Pipette, 1000 – 5000 µl, fully autoclavable, 1022712 [W161491], Pipets and Micropipets](/thumblibrary/W161491/W161491_01_140_140_Microlitre-Pipette-1000-5000-µl-fully-autoclavable.jpg)
![Pipette tips, up to 5000 µl, 250 pcs/pkg, 1023411 [W16205], Pipets and Micropipets](/thumblibrary/W16205/W16205_01_140_140_Pipette-tips-up-to-5000-µl-250-pcspkg.jpg)
![AB0/Rh Blood Typing Refill kit, 1022413 [W55013], Genetics Experiments](/thumblibrary/W55013/W55013_01_140_140_AB0Rh-Blood-Typing-Refill-kit.jpg)
![Osmosis and Diffusion Lab, 1022429 [W56614], Diffusion and Osmosis Experiments](/thumblibrary/W56614/W56614_01_140_140_Osmosis-and-Diffusion-Lab.jpg)
![Electrophoresis: Agarose Gel Separation of Dyes, 1022410 [W56628], Electrophoresis Experiments](/thumblibrary/W56628/W56628_01_140_140_Electrophoresis-Agarose-Gel-Separation-of-Dyes.jpg)
![Experiment Set “Conduction of Impulses to Nerve Fibres”, 1000538 [U11120], Nervous System Models](/thumblibrary/U11120/U11120_01_140_140_Experiment-Set-Conduction-of-Impulses-to-Nerve-Fibres.jpg)
![pH meter (2 in 1), 1020914 [U11838], pH Measuring](/thumblibrary/U11838/U11838_01_140_140_pH-meter-2-in-1.jpg)
![Microlitre Pipette, 10 – 100 µl, fully autoclavable, 1022708 [W161901], Pipets and Micropipets](/thumblibrary/W161901/W161901_01_140_140_Microlitre-Pipette-10-100-µl-fully-autoclavable.jpg)
![Pipettenspitzen, Blue, bis 1000 µl, 1013426 [W16195], Pipets and Micropipets](/thumblibrary/W16195/W16195_01_140_140_Pipettenspitzen-Blue-bis-1000-µl.jpg)
![Chemistry of Who done it? Lab - Crime Scene Investigation Lab, 1022423 [W56610], Forensics Experiments](/thumblibrary/W56610/W56610_01_140_140_Chemistry-of-Who-done-it-Lab-Crime-Scene-Investigation-Lab.jpg)
![Microlitre Pipette, 20 - 200 µl, fully autoclavable, 1022709 [W161911], Pipets and Micropipets](/thumblibrary/W161911/W161911_01_140_140_Microlitre-Pipette-20-200-µl-fully-autoclavable.jpg)